报告题目：Seed mixture refuge strategy and Vip3A resistance in fall armyworm

时 间： 2018年1月9日下午3点

地 点： 生物楼N406

报告简介：

    Fall armyworm (FAW), Spodoptera frugiperda, and corn earworm (CEW), Helicoverpa zea, are two target pests of pyramided Bt corn in the U.S. This study first determined if seed mixture of Bt and non-Bt corn was an appropriate approach to provide refuge populations of ear-feeding insects for resistance management. First, intensity of Bt protein contamination and its associated impacts on CEW populations were assessed in four different mixed plantings of 5:95% RIB (non-Bt: Bt), 10: 90% RIB, 20:80% RIB, and 30:70% RIB corn with the SmartStax^TM traits. Results of field and laboratory studies revealed that cross-pollinations in the mixed planting caused a majority (> 90%) of refuge kernels to express ≥ one Bt protein. The intensive Bt protein contamination in refuge ears significantly reduced neonate-to-adult survivorship of refuge populations of CEW. Second, we used FAW and Cry1F-corn plants as a model to examine if pollen contamination in seed mixtures increases the dominance of resistance in two different mixed plantings of 5:95% RIB and 20:80% RIB. The results showed that pollen contamination in RIBs could increase the dominance of resistance for ear-feeding insects by significantly reducing susceptible refuge populations and supporting higher survival of heterozygotes relative to homozygous susceptible insects. The results together suggest that seed mixtures strategy is not effective for resistance management of ear-feeding insects. Second, we provide the first documentation of a resistance allele conferring Vip3A resistance in a field-derived population of FAW from U.S., and characterize its inheritance and cross-resistance. The results showed that the inheritance of Vip3A resistance was monogenic, autosomal and recessive. The Vip3A resistance conferred no cross-resistance to Cry1F, Cry2Ab2 or Cry2Ae proteins. These findings provide valuable information for characterizing Vip3A resistance, resistance monitoring, and developing effective resistance management strategies for the sustainable use of the Vip3A technology.

杨飞博士简介：

   杨飞，美国路易斯安那州立大学博士，美国路易斯安那州立大学博士后，目前在美国德州农工大学任助理研究员。主要研究领域为田间农作物、蔬菜、水果等作物与昆虫生物学、行为学、生态学及分子生物学，致力于研究利用昆虫学基础与应用数据来发现昆虫与植物间的关系，提高农业产量和农业生产力。